Difference Between PCR VS REAL TIME PCR

pcr vs real time pcr

PCR means “polymerase chain reaction” that is based on the instinctive process of DNA replication, and is a tool that is used to amplify and simultaneously detect DNA’s and having it copied billion of times over. This PCR, developed by Kary Mullis in 1980’s, is a great discovery that is still being used nowadays. With the use of PCR, it is now possible to determine the amount of viruses in a human body. Various diseases can now also be detected through PCR. The PCR process has three stages which are exponential, linear, and plateau. In PCR, data’s are gathered at the end-point or plateau phase, making the quantification data unreliable.

Real Time PCR involves genetics and molecular biology techniques, which are compelling and delicate, that is based on PCR. It can be used in many fields and wide extent of applications including the quantitative measurements of gene transcription. The procedure of Real time PCR also follows the general principle of polymerase reaction. The products for a Real time PCR can be determined in two methods: namely the (1)non-specific fluorescent dyes, and (2) sequence-specific DNA probes. Unlike the PCR that was first described above, Real time PCR collects data in the exponential growth phase of PCR, making it more reliable than that of the PCR data.

Advertisement

No comments.

Leave a Reply